PromethION sequencer

This Request for Quotation (RFQ) seeks a long-read sequencing instrument capable of producing 90 GB of sequencing data from large genomes (minimum 20kb fragments), with the ability to sequence smaller fragments for transcriptome studies. The instrument must detect 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) modifications and output data in FASTQ and/or BAM formats. On-site computing eliminates the need for integrated computer infrastructure. Training and reagents for training are required. The 90-day performance period begins upon contract award. The USDA/ARS/USNPRC in Athens, GA will provide facility access (Monday-Friday, 7:30 am-4 pm) and a delivery location. Offerors must complete FAR Provision 52.212-3, Offeror Representations and Certifications—Commercial Items (DEVIATION), online via SAM.gov and reference completion in their quote. Quotes are due September 17, 2025, at 5:00 PM EST to peter.b.nelson@usda.gov. Evaluation criteria are detailed in the attached RFQ terms and conditions.

Notice ID

31bdf06d0fd14fc0961043778a7d5fe4

Solicitation Number

12405b25q0167

NAICS Code

334516

Classification Code

6640